Recombinant Anti-HIV1 p25 Antibody (V3S-0522-YC3317) (CAT#: V3S-0522-YC3317)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Human immunodeficiency virus type 1 major core protein p25. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human immunodeficiency virus 1 (HIV1)
Immunogen Recombinant HIV core antigen p25
Isotype IgG1, κ

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; ADCC
Application Notes The antibody is recommended for detection of HIV1 p25 by ELISA, ADCC assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target HIV1 p25
Alternative Name Human immunodeficiency virus type 1; HIV-1; major core protein p25; p25
Gene ID 155030
UniProt P04591
Research Area Microbiology; Immunology
Related Disease AIDS
For research use only, not directly for clinical use.
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