Description | This product is a recombinant Mouse antibody provided by CreativeBiolabs that react with gD of HSV-1 and HSV-2. It can be used for HSV gD detection in Neutralization Assay (Neut), Immunohistochemistry (IHC) and Western Blot (WB). It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
Clonality | Monoclonal |
Host Species | Mouse |
Target Species | Herpes simplex virus 1 (HSV1), Herpes simplex virus 2 (HSV2) |
Immunogen | Whole Herpex Simplex Virus 2 (HSV-2) |
Epitope | Located in the sequence: MADPNRFRG. |
Isotype | IgG2a kappa |
Expression Species | HEK293F or CHO Cell line |
Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
Purity | >90% |
Endotoxin | <1 EU/mg, determined by LAL method |
Purification | Purified with Protein G affinity chromatography |
Sterility | 0.2 μM filtered |
Formulation | PBS |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
Application | Neut; IHC; WB |
Application Notes | It has shown that the antibody neutralized 80% of the virus at a dilution of 1:30. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |