Anti-IAV HA Neutralizing Antibody (V3S-0522-YC7447) (CAT#: V3S-0522-YC7447)

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  • fig1
    Figure 1 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in SDS-PAGE
  • fig1
    Figure 2 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in SEC-HPLC
  • fig1
    Figure 3 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in ELISA
  • fig1
    Figure 4 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in DB

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Influenza A virus hemagglutinin. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Influenza A virus (IAV)
Immunogen Influenza a virus Hemagglutinin
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; DB; ELISA; Neut; FuncS
Application Notes The antibody is recommended for detection of IAV HA by ELISA, Neut, FuncS assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target IAV HA
Alternative Name HA; hemagglutinin; haemagglutinin
Research Area Microbiology; Infectious Disease

Tested Data

SDS-PAGE

Figure 1 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in SDS-PAGE

SDS-PAGE analysis of V3S-0522-YC7447 in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 135-180 kDa.

Figure 1 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in SDS-PAGE

SEC-HPLC

Figure 2 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in SEC-HPLC

SEC-HPLC analysis of V3S-0522-YC7447 shows the purity is more than 95%.

Figure 2 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in SEC-HPLC

ELISA

Figure 3 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in ELISA

ELISA analysis of V3S-0522-YC7447 was performed by coating with Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with V3S-0522-YC7447. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 3 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in ELISA

DB

Figure 4 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in DB

Dot Blot analysis of V3S-0522-YC7447 was performed by coating with Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag).

V3S-0522-YC7447 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)

Figure 4 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in DB

For research use only, not directly for clinical use.
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