Anti-IAV HA Neutralizing Antibody (V3S-0522-YC7447) (CAT#: V3S-0522-YC7447)

Figure 1 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in SDS-PAGE

SDS-PAGE analysis of V3S-0522-YC7447 in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 135-180 kDa.

Figure 2 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in SEC-HPLC

SEC-HPLC analysis of V3S-0522-YC7447 shows the purity is more than 95%.

Figure 3 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in ELISA

ELISA analysis of V3S-0522-YC7447 was performed by coating with Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with V3S-0522-YC7447. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 4 Anti-Influenza A Virus HA Monoclonal Antibody (V3S-0522-YC7447) in DB

Dot Blot analysis of V3S-0522-YC7447 was performed by coating with Influenza A H2N2 (A/Japan/305/1957) Hemagglutinin / HA Protein (His Tag).

V3S-0522-YC7447 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)