| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Influenza A virus hemagglutinin. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Influenza A virus (IAV), Influenza B virus (IBV) |
| Immunogen | Influenza a virus Hemagglutinin |
| Isotype | IgG, λ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; DB; Neut; FuncS |
| Application Notes | The antibody is recommended for detection of IAV HA by Neut, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | IAV HA |
| Alternative Name | HA; hemagglutinin; haemagglutinin |
| Research Area | Microbiology; Infectious Disease |
Figure 1 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in SDS-PAGE
SDS-PAGE analysis of V3S-0522-YC7450 in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180 kDa.
Figure 1 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in SDS-PAGE
Figure 2 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in SEC-HPLC
The purity of V3S-0522-YC7450 was greater than 99% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 2 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in SEC-HPLC
Figure 3 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in ELISA
ELISA analysis of V3S-0522-YC7450 was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with V3S-0522-YC7450. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in ELISA
Figure 4 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in ELISA
ELISA analysis of V3S-0522-YC7450 was performed by coating with Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) Protein (His Tag). Then blocked with BSA and incubated with V3S-0522-YC7450. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in ELISA
Figure 5 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in WB
Western blot analysis of V3S-0522-YC7450 was performed by loading Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC7450 and HRP goat anti-human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
Figure 5 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in WB
Figure 6 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in WB
Western blot analysis of V3S-0522-YC7450 was performed by loading Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC7450 and HRP goat anti-human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
Figure 6 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in WB
Figure 7 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in DB
Dot Blot analysis of V3S-0522-YC7450 was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag).
V3S-0522-YC7450 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000)
Figure 7 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in DB
Figure 8 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in DB
Dot Blot analysis of V3S-0522-YC7450 was performed by coating with Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) Protein (His tag).
V3S-0522-YC7450 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000)
Figure 8 Anti-IAV HA Monoclonal Antibody (V3S-0522-YC7450) in DB
Figure 9 Anti-IAV HA Neutralizing Antibody (V3S-0522-YC7450) in Dot Blot.
Dot blot analysis of V3S-0522-YC7450 was performed with one of the conserved neutralizing epitopes on influenza A and B, recombinant influenza A H5N1 (A/Vietnam/1194/2004) Hemagglutinin / HA protein (His Tag).
The secondary antibody: HRP goat anti-human IgG (H+L).
Figure 9 Anti-IAV HA Neutralizing Antibody (V3S-0522-YC7450) in Dot Blot.
Figure 10 Anti-IAV HA Neutralizing Antibody (V3S-0522-YC7450) in ELISA.
ELISA analysis of V3S-0522-YC7450 was performed by coating with with one of the conserved neutralizing epitopes on influenza A and B, recombinant influenza A H5N1 (A/Vietnam/1194/2004) Hemagglutinin / HA protein (His Tag).
The secondary antibody: HRP goat anti-human IgG (H+L).
Figure 10 Anti-IAV HA Neutralizing Antibody (V3S-0522-YC7450) in ELISA.
