| Description | This product is a human monoclonal antibody that reacts with IFNAR1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | FuncS; IF; Neut; ELISA; FC; IP; IHC |
| Application Notes | The antibody is recommended for detection of IFNAR1 by FuncS, IF, Neut, ELISA, FC, IP, IHC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-IFNAR1 Neutralizing Antibody(V3S-0622-YC4567) in SDS-PAGE.
SDS-PAGE analysis of V3S-0622-YC4567 in β-mercaptoethanol-reduced (Lane 1, 1.5 μg) and non-reduced (Lane 2, 1.5 μg) conditions.
Figure 1 Anti-IFNAR1 Neutralizing Antibody(V3S-0622-YC4567) in SDS-PAGE.
Figure 2 Anti-IFNAR1 Neutralizing Antibody(V3S-0622-YC4567) in SEC-HPLC.
The purity of V3S-0622-YC4567 was greater than 95% as determined by SEC-HPLC.
Figure 2 Anti-IFNAR1 Neutralizing Antibody(V3S-0622-YC4567) in SEC-HPLC.
Figure 3 Anti-IFNAR1 Neutralizing Antibody(V3S-0622-YC4567) in ELISA.
ELISA analysis of V3S-0622-YC4567 was performed by coating with recombinant IFNAR protein, His tag.
Figure 3 Anti-IFNAR1 Neutralizing Antibody(V3S-0622-YC4567) in ELISA.
