| Description | This product is a mouse monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing interferon beta 1. It can be used for IFNB1 detection in Western Blot (WB), Enzyme-Linked Immunosorbent Assay (ELISA), Neutralization Assay (Neut). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Recombinant HuIFN-β1b |
| Isotype | IgG1 kappa |
| Conjugation | Unconjugated |
| Purity | >95%, determined by SDS-PAGE |
| Purification | Affinity purified |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | DB; WB; ELISA; Neut |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Mouse Anti-IFNB1 Monoclonal Antibody (V3S-0822-YC1351) in ELISA
ELISA analysis of V3S-0822-YC1351 was performed by coating with human IFN-beta protein (His tag). Then blocked with BSA and incubated with serial dilutions of V3S-0822-YC1351. The HRP conjugated anti-mouse IgG as a secondary antibody diluted at 1/2000.
Figure 1 Mouse Anti-IFNB1 Monoclonal Antibody (V3S-0822-YC1351) in ELISA
Figure 2 Mouse Anti-IFNB1 Monoclonal Antibody (V3S-0822-YC1351) in WB
Western blot analysis of V3S-0822-YC1351 was performed by loading human IFN-beta protein, His tag.
The incubation concentration of V3S-0822-YC1351 is 2 ng/μL.
The secondary antibody: HRP-Goat anti-mouse IgG (H+L).
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 2: Reduced antigen (0.6 μg)
Figure 2 Mouse Anti-IFNB1 Monoclonal Antibody (V3S-0822-YC1351) in WB
Figure 3 Mouse Anti-IFNB1 Monoclonal Antibody (V3S-0822-YC1351) in DB
Dot Blot analysis of V3S-0822-YC1351 was performed by coating with human IFN-beta protein (His tag).
The incubation concentration of V3S-0822-YC1351 is 2 μg/mL.
HRP conjugated anti-mouse IgG as a secondary antibody diluted at 1/2000.
Figure 3 Mouse Anti-IFNB1 Monoclonal Antibody (V3S-0822-YC1351) in DB
