Recombinant Anti-IGE-M1 Antibody (V3S-1022-YC1003) (CAT#: V3S-1022-YC1003)

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Datasheet

MSDS

COA

Sub Cat Application Affinity  
V3S-1124-YC587 ELISA; FC 5.1 nM Inquiry
V3S-1022-YC1004 ELISA; FC 4.5 nM Inquiry
V3S-1022-YC1005 Inhib; FC; ELISA 1.5 nM Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant human antibody provided by CreativeBiolabs. The antibody is specific for Immunoglobulin E, Immunoglobulin E (IgE) are antibodies produced by the immune system. It can be used for IGE-M1 detection in Enzyme-Linked Immunosorbent Assay, Flow Cytometry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Human
Target Species Human
Affinity The affinity is in nanomolar range, about 5.1 nM, determined by surface plasmon resonance.
Isotype IgE

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application ELISA; FC

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target IGE-M1
Alternative Name Immunoglobulin E/M1; IGE-M1
For research use only, not directly for clinical use.
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