| Sub Cat | Epitope | Isotype | Application | Affinity | |
|---|---|---|---|---|---|
| V3S-1124-YC297 | IgG1 kappa | ELISA; Inhib | |||
| V3S-0522-YC8154 | The CεmX domain of human membrane-bound IgE. | IgG | Neut; FuncS | ||
| V3S-0522-YC7466 | The antibody inhibits the interaction between IgE-Fc and its two receptors21; binding to FcεRI is inhibited due to steric overlap, while binding to CD23 is inhibited as the Cε3 domains are "locked" in an open conformation. | IgG1 | WB; ELISA; Neut; FuncS | ||
| V3S-0522-YC2325 | The antibody binds to IgE on the same Fc site as FcεRI. | IgG1 | ELISA; FuncS | 0.2 nM | |
| V3S-0522-YC2326 | The antibody binds to IgE on the same Fc site as FcεRI. | IgG1 | ELISA; FuncS | 61 pM | |
| V3S-0522-YC2327 | The antibody binds to IgE on the same Fc site as FcεRI. | IgG1 | ELISA; FuncS | 64 pM | |
| V3S-0522-YC2315 | IgG | ELISA | |||
| V3S-0622-YC1763 | IgG | ELISA | |||
| V3S-0622-YC3463 | IgG1 | ELISA; WB |
| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Immunoglobulin E. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG1, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; Inhib |
| Application Notes | The antibody is recommended for detection of IgE by ELISA, Inhib assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | IgE |
| Alternative Name | Immunoglobulin E |
| Research Area | Immunology |
| Related Disease | Allergic Asthma, Urticaria |
