Recombinant Anti-IGF-I/IGF-II Antibody (V3S-1022-YC1020) (CAT#: V3S-1022-YC1020)

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Datasheet

MSDS

COA

Sub Cat Target Species Affinity  
V3S-1124-YC1610 Human, Mouse 60 pM Inquiry
V3S-1022-YC1019 Human Inquiry
V3S-1022-YC1021 Human 60 pM Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant human antibody provided by CreativeBiolabs. The antibody is specific for Insulin Like Growth Factor 1, the protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. It can be used for IGF-I / IGF-II detection in Enzyme-Linked Immunosorbent Assay. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU / mg or even 0.1 EU / mg.
Clonality Monoclonal
Host Species Human
Target Species Human, Mouse
Affinity The affinity is in picomolar range, about 60 pM, determined by surface plasmon resonance.
Isotype IgG

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application ELISA

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target IGF-I/IGF-II
Alternative Name Insulin Like Growth Factor 1
Gene ID 3479
UniProt P05019
Research Area Signal Pathway; Cancer Research
For research use only, not directly for clinical use.
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