Description | This product is a human monoclonal antibody that reacts with IL12/IL23. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | Human |
Isotype | IgG |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
Endotoxin | <1 EU/mg, determined by LAL method |
Purification | Protein A affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
Application | WB; FC; IP; ELISA; Neut; FuncS; IF |
Application Notes | The antibody is recommended for detection of IL12/IL23 by WB, FC, IP, ELISA, Neut, FuncS, IF assays. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | IL12/IL23 |
Alternative Name | IL12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); NKSF2; interleukin-12 subunit beta; CLMF; CLMF2; cytoto |
Research Area | Cell Biology |
Figure 1 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Western Blot.
Western blot analysis of V3S-0622-YC1812 was performed by loading human IL12 protein (His Tag).
Figure 1 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Western Blot.
Figure 2 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Western Blot.
Western blot analysis of V3S-0622-YC1812 was performed by loading human IL23 protein (His Tag).
Figure 2 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Western Blot.
Figure 3 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in ELISA.
ELISA analysis of V3S-0622-YC1812 was performed by coating with human IL12 protein (His Tag).
Figure 3 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in ELISA.
Figure 4 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in ELISA.
ELISA analysis of V3S-0622-YC1812 was performed by coating with human IL23 protein (His Tag).
Figure 4 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in ELISA.
Figure 5 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Dot Blot.
Dot blot analysis of V3S-0622-YC1812 was performed by coating with human IL12 protein (His Tag).
Figure 5 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Dot Blot.
Figure 6 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Dot Blot.
Dot blot analysis of V3S-0622-YC1812 was performed by coating with human IL23 protein (His Tag).
Figure 6 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Dot Blot.