Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) (CAT#: V3S-0622-YC1812)

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  • fig1
    Figure 1 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Western Blot.
  • fig1
    Figure 2 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Western Blot.
  • fig1
    Figure 3 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in ELISA.
  • fig1
    Figure 4 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in ELISA.
  • fig1
    Figure 5 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Dot Blot.
  • fig1
    Figure 6 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Dot Blot.

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a human monoclonal antibody that reacts with IL12/IL23. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application WB; FC; IP; ELISA; Neut; FuncS; IF
Application Notes The antibody is recommended for detection of IL12/IL23 by WB, FC, IP, ELISA, Neut, FuncS, IF assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target IL12/IL23
Alternative Name IL12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); NKSF2; interleukin-12 subunit beta; CLMF; CLMF2; cytoto
Research Area Cell Biology

Tested Data

WB

Figure 1 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Western Blot.

Western blot analysis of V3S-0622-YC1812 was performed by loading human IL12 protein (His Tag).

Figure 1 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Western Blot.

WB

Figure 2 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Western Blot.

Western blot analysis of V3S-0622-YC1812 was performed by loading human IL23 protein (His Tag).

Figure 2 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Western Blot.

ELISA

Figure 3 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in ELISA.

ELISA analysis of V3S-0622-YC1812 was performed by coating with human IL12 protein (His Tag).

Figure 3 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in ELISA.

ELISA

Figure 4 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in ELISA.

ELISA analysis of V3S-0622-YC1812 was performed by coating with human IL23 protein (His Tag).

Figure 4 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in ELISA.

DB

Figure 5 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Dot Blot.

Dot blot analysis of V3S-0622-YC1812 was performed by coating with human IL12 protein (His Tag).

Figure 5 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Dot Blot.

DB

Figure 6 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Dot Blot.

Dot blot analysis of V3S-0622-YC1812 was performed by coating with human IL23 protein (His Tag).

Figure 6 Anti-IL12/IL23 Neutralizing Antibody (V3S-0622-YC1812) in Dot Blot.

For research use only, not directly for clinical use.
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