| Description | This product is a monoclonal antibody derived from rat, which can specifically recognize IL1R1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Rat |
| Target Species | Mouse |
| Immunogen | Mouse ST2-Fc |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; DB; ELISA; Inhib; FC |
| Application Notes | The antibody is recommended for detection of IL1R1 by ELISA, Inhib, FC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-IL1RL1 Monoclonal Antibody (V3S-0622-YC4632) in WB
Western blot analysis of V3S-0622-YC4632 was performed by loading Mouse IL1RL1 Protein (His Tag) in reduced condition Lane 1, Lane 2, Lane 3 (0.1 μg, 0.3 μg, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC4632 (2 μg/mL) and HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
Figure 1 Anti-IL1RL1 Monoclonal Antibody (V3S-0622-YC4632) in WB
Figure 2 Anti-IL1RL1 Monoclonal Antibody (V3S-0622-YC4632) in DB
Dot Blot analysis of V3S-0622-YC4632 was performed by coating with Mouse IL1RL1 Protein (His Tag).
V3S-0622-YC4632 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 6000)
Figure 2 Anti-IL1RL1 Monoclonal Antibody (V3S-0622-YC4632) in DB
Figure 3 Anti-IL1RL1 Monoclonal Antibody (V3S-0622-YC4632) in ELISA
ELISA analysis of V3S-0622-YC4632 was performed by coating with Mouse IL1RL1 Protein (His Tag). Then blocked with BSA and incubated with V3S-0622-YC4632. The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-IL1RL1 Monoclonal Antibody (V3S-0622-YC4632) in ELISA
