| Description | This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for Interleukin 21. It can be used for Interleukin 21 detection in Enzyme-Linked Immunosorbent Assay and Neutralization Assay. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Cynomolgus |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | WB; DB; ELISA; Neut |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-IL21 Monoclonal Antibody (V3S-1022-YC2991) in WB
Western blot analysis of V3S-1022-YC2991 was performed by loading human IL21 protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-1022-YC2991 and HRP goat anti-human IgG as a secondary antibody (1: 3000). Chemiluminescent detection was performed.
Figure 1 Anti-IL21 Monoclonal Antibody (V3S-1022-YC2991) in WB
Figure 2 Anti-IL21 Monoclonal Antibody (V3S-1022-YC2991) in DB
Dot Blot analysis of V3S-1022-YC2991 was performed by coating with human IL21 protein (His tag).
V3S-1022-YC2991 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 3000)
Figure 2 Anti-IL21 Monoclonal Antibody (V3S-1022-YC2991) in DB
Figure 3 Anti-IL21 Monoclonal Antibody (V3S-1022-YC2991) in ELISA
ELISA analysis of V3S-1022-YC2991 was performed by coating with human IL21 protein (His tag). Then blocked with BSA and incubated with V3S-1022-YC2991. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 4000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-IL21 Monoclonal Antibody (V3S-1022-YC2991) in ELISA
