-1-1.jpg)
-1-2.jpg)
-1-3.jpg)
| Description | This product is a human monoclonal antibody that reacts with IL21. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; FC |
| Application Notes | The antibody is recommended for detection of IL21 by ELISA, FC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Recombinant Anti-IL21 Antibody (V3S-0622-YC4656) in Western Blot.
Western blot analysis of V3S-0622-YC4656 was performed by loading human IL21 protein.
Figure 1 Recombinant Anti-IL21 Antibody (V3S-0622-YC4656) in Western Blot.
Figure 2 Recombinant Anti-IL21 Antibody (V3S-0622-YC4656) in ELISA.
ELISA analysis of V3S-0622-YC4656 was performed by coating with human IL21 protein.
Figure 2 Recombinant Anti-IL21 Antibody (V3S-0622-YC4656) in ELISA.
Figure 3 Recombinant Anti-IL21 Antibody (V3S-0622-YC4656) in Dot Blot.
Dot blot analysis of V3S-0622-YC4656 was performed by coating with human IL21 protein.
Figure 3 Recombinant Anti-IL21 Antibody (V3S-0622-YC4656) in Dot Blot.
Figure 4 Recombinant Anti-IL21 Antibody (V3S-0622-YC4656) Fab Fragment in Western Blot.
Western blot analysis of V3S-0622-YC4656-Fab was performed by loading human IL21 protein.
Figure 4 Recombinant Anti-IL21 Antibody (V3S-0622-YC4656) Fab Fragment in Western Blot.
Figure 5 Recombinant Anti-IL21 Antibody (V3S-0622-YC4656) Fab Fragment in ELISA.
ELISA analysis of V3S-0622-YC4656-Fab was performed by coating with human IL21 protein.
Figure 5 Recombinant Anti-IL21 Antibody (V3S-0622-YC4656) Fab Fragment in ELISA.
Figure 6 Recombinant Anti-IL21 Antibody (V3S-0622-YC4656) Fab Fragment in Dot Blot.
Dot blot analysis of V3S-0622-YC4656-Fab was performed by coating with human IL21 protein.
Figure 6 Recombinant Anti-IL21 Antibody (V3S-0622-YC4656) Fab Fragment in Dot Blot.
