| Description | This product is a human monoclonal antibody that reacts with IL6R. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | IL-6R bound to Sepharose4B |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; FC; IP; FuncS; IF; Neut; ICC |
| Application Notes | The antibody is recommended for detection of IL6R by ELISA, FC, IP, FuncS, IF, Neut, ICC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in SDS-PAGE
SDS-PAGE analysis of V3S-0622-YC1944 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.
Figure 1 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in SDS-PAGE
Figure 2 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in SEC-HPLC
The purity of V3S-0622-YC1944 was greater than 95% as determined by SEC-HPLC.
Figure 2 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in SEC-HPLC
Figure 3 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in WB
Western blot analysis of V3S-0622-YC1944 was performed by loading Recombinant Human IL6R protein.
Lane 1: Non-Reducing Antigen (1 μg)
Lane 2: Reducing Antigen (1 μg)
Figure 3 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in WB
Figure 4 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in ELISA
ELISA analysis of V3S-0622-YC1944 was performed by coating with Recombinant Human IL6R protein.
Figure 4 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in ELISA
Figure 5 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in ELISA
ELISA analysis of V3S-0622-YC1944 was performed by coating with Recombinant Human IL6R Protein.
Figure 5 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in ELISA
