Anti-IL6R Neutralizing Antibody (V3S-0622-YC1944)

Figure 1 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in SDS-PAGE
Figure 2 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in SEC-HPLC
Figure 3 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in WB
Figure 4 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in ELISA
Figure 5 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in ELISA

Datasheet

MSDS

COA

Summary

Description	This product is a human monoclonal antibody that reacts with IL6R. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality	Monoclonal
Host Species	Human
Target Species	Human
Immunogen	IL-6R bound to Sepharose4B
Isotype	IgG

Tested Data

SDS-PAGE

Figure 1 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in SDS-PAGE

SDS-PAGE analysis of V3S-0622-YC1944 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.

Figure 1 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in SDS-PAGE

SEC-HPLC

Figure 2 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in SEC-HPLC

The purity of V3S-0622-YC1944 was greater than 95% as determined by SEC-HPLC.

Figure 2 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in SEC-HPLC

Figure 3 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in WB

Western blot analysis of V3S-0622-YC1944 was performed by loading Recombinant Human IL6R protein.

Lane 1: Non-Reducing Antigen (1 μg)
Lane 2: Reducing Antigen (1 μg)

Figure 3 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in WB

ELISA

Figure 4 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in ELISA

ELISA analysis of V3S-0622-YC1944 was performed by coating with Recombinant Human IL6R protein.

Figure 4 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in ELISA

ELISA

Figure 5 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in ELISA

ELISA analysis of V3S-0622-YC1944 was performed by coating with Recombinant Human IL6R Protein.

Figure 5 Anti-IL6R Monoclonal Antibody (V3S-0622-YC1944) in ELISA

Expression Species	HEK293F or CHO
Conjugation	None
Purity	>95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin	<1 EU/mg, determined by LAL method
Purification	Protein A affinity purified
Sterility	0.2 μM filtered
Formulation	PBS, pH 7.4
Preservation	No preservatives
Stabilizer	No stabilizers
Storage	Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Application	WB; ELISA; FC; IP; FuncS; IF; Neut; ICC
Application Notes	The antibody is recommended for detection of IL6R by ELISA, FC, IP, FuncS, IF, Neut, ICC assays.

ELISA	Enzyme-Linked Immunosorbent Assay Protocol
WB	Western Blot Protocol
FC	Flow Cytometry Protocol

Target	IL6R
Alternative Name	IL6R; interleukin 6 receptor; interleukin-6 receptor subunit alpha; CD126; IL-6R 1; CD126 antigen; membrane glycoprotein 80; IL-6 receptor subunit alpha; gp80; IL6RA; IL-6RA; IL-6R-1
Gene ID	3570
UniProt	P08887
Research Area	Immunology

Anti-IL6R Neutralizing Antibody (V3S-0622-YC1944) (CAT#: V3S-0622-YC1944)

Summary

Property

Applications

Protocols

Target

Tested Data

Anti-IL6R Neutralizing Antibody (V3S-0622-YC1944) (CAT#: V3S-0622-YC1944)

Summary

Property

Applications

Protocols

Target

Tested Data

Isotype Control

Secondary Antibody

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