Anti-IL7R Neutralizing Antibody (V3S-1022-YC3103) (CAT#: V3S-1022-YC3103)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant Rat antibody provided by CreativeBiolabs. The antibody is specific for Interleukin-7 receptor. It can be used for Interleukin-7 receptor detection in Enzyme-Linked Immunosorbent Assay, Flow Cytometry and Blocking. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Rat
Target Species Human, Cynomolgus, Baboon
Immunogen Recombinant hCD127-Ig (hCD127 fused with a constant fragment of an immunoglobulin)
Affinity The affinity is in nanomolar range, about 6.5 nM, determined by surface plasmon resonance.
Isotype IgG2a

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application ELISA; FC; Block

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target IL7R
Alternative Name ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
Gene ID 3575
UniProt P16871
Research Area Immunology; Stem Cell; Cancer Research
Related Disease Cancer
For research use only, not directly for clinical use.
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