Recombinant Anti-MADCAM1 Antibody (V3S-0522-YC7828) (CAT#: V3S-0522-YC7828)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Mucosal Addressin Cell Adhesion Molecule 1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Immunogen Human mucosal vascular addressin cell adhesion molecule 1
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application WB; IF; FuncS
Application Notes The antibody is recommended for detection of MADCAM1 by WB (1/1000-1/10000), IF, FuncS assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target MADCAM1
Alternative Name MACAM1; MADCAM1; mucosal vascular addressin cell adhesion molecule 1
Gene ID 8174
UniProt Q13477
Research Area Cardiovascular; Immunology
For research use only, not directly for clinical use.
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