| Sub Cat | Immunogen | Epitope | Isotype | Application | |
|---|---|---|---|---|---|
| V3S-1124-YC810 | The extracellular domain of human MCAM with human IgG Fc and produced in CHO cells | In the ECD domain. | IgG | ELISA; Block | |
| V3S-0622-YC363 | MCAM-Fc | Located in extracellular domain, the combination of human MCAM domain 1 (namely aa 19-129), and domain 2 (namely aa 139-242). | IgG1 kappa | FC; Neut; IF; ELISA |
| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Melanoma Cell Adhesion Molecule. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | The extracellular domain of human MCAM with human IgG Fc and produced in CHO cells |
| Epitope | Within the ECD domain. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; Block |
| Application Notes | The antibody is recommended for detection of MCAM by ELISA, Block assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
