| Description | This product is a monoclonal antibody derived from rat, which can specifically recognize MCAM. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Rat |
| Target Species | Human |
| Immunogen | MCAM-Fc |
| Epitope | Located in extracellular domain, the combination of human MCAM domain 1 (namely aa 19-129), and domain 2 (namely aa 139-242). |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | FC; Neut; IF; ELISA |
| Application Notes | The antibody is recommended for detection of MCAM by FC, Neut, IF (1/100-1/500), ELISA assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | MCAM |
| Alternative Name | CD146; MUC18; HEMCAM; METCAM; MelCAM; cell surface glycoprotein MUC18; Gicerin; S-endo 1 endothelial-associated antigen; cell surface glycoprotein P1H12; melanoma adhesion molecule; melanoma-associated antigen A32; melanoma-associated antigen MUC18 |
| Gene ID | 4162 |
| UniProt | P43121 |
| Research Area | Immunology; Signal Pathway |
