Anti-MCAM (Ig domain 3) Neutralizing Antibody (V3S-0622-YC364) (CAT#: V3S-0622-YC364)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from mouse, which can specifically recognize MCAM. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Immunogen MCAM-Fc
Epitope The epitope is a conformationally complex epitope at the third Ig domain.
Affinity 0.286 nM
Isotype IgG1 kappa

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application FC; Neut; IF; ELISA
Application Notes The antibody is recommended for detection of MCAM by FC, Neut, IF (1/100-1/500), ELISA assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target MCAM
Alternative Name CD146; MUC18; HEMCAM; METCAM; MelCAM; cell surface glycoprotein MUC18; Gicerin; S-endo 1 endothelial-associated antigen; cell surface glycoprotein P1H12; melanoma adhesion molecule; melanoma-associated antigen A32; melanoma-associated antigen MUC18
Gene ID 4162
UniProt P43121
Research Area Immunology; Signal Pathway
For research use only, not directly for clinical use.
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