| Description | This product is a broadly neutralizing mouse antibody against MERS-CoV S protein. It binds to the N-terminal domain (NTD) of the spike glycoprotein and inhibits the cell entry of MERS-CoV with high potency. The 7D10 bound the wild-type NTD with an affinity of around 25 nM. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Middle East Respiratory Syndrome Coronavirus (MERS-CoV) |
| Epitope | N-terminal |
| IC50 | 0.18 μg/mL |
| EC50 | 0.31 μg/mL |
| Isotype | Mouse IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Neut; WB; FC |
| Application Notes | The NTD-specific monoclonal antibody, 7D10, was successfully isolated with an EC₅₀ of roughly 0.31 μg/mL in ELISA. Its neutralizing activity was dose-dependent, exhibiting an IC₅₀ of about 0.18 μg/mL against pseudotyped virus and a comparable IC50 of around 0.2 μg/mL against live virus (EMC strain). |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | MERS-CoV S |
| Alternative Name | MERS-CoV S Glycoprotein; MERS-CoV; S Glycoprotein; spike; MERS |
| Research Area | Microbiology |
