| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize MET proto-oncogene, receptor tyrosine kinase. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG1, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; Block; FuncS |
| Application Notes | The antibody is recommended for detection of MET by WB, Block, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | MET |
| Alternative Name | MET; MET proto-oncogene; receptor tyrosine kinase; HGFR; AUTS9; RCCP2; c-Met; hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase |
| Gene ID | 4233 |
| UniProt | A0A024R759 |
| Research Area | Signal Pathway; Epigenetics |
Figure 1 Human Anti-MET Recombinant Antibody (V3S-0522-YC7505) in Western Blot.
Western blot analysis of V3S-0522-YC7505 was performed by loading recombinant human Met.
V3S-0522-YC7505 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-anti-human IgG
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 1 Human Anti-MET Recombinant Antibody (V3S-0522-YC7505) in Western Blot.
Figure 2 Human Anti-MET Recombinant Antibody (V3S-0522-YC7505) in ELISA.
ELISA analysis of V3S-0522-YC7505 was performed by coating with recombinant human Met.
The secondary antibody: HRP-anti-human IgG
Figure 2 Human Anti-MET Recombinant Antibody (V3S-0522-YC7505) in ELISA.
Figure 3 Human Anti-MET Recombinant Antibody (V3S-0522-YC7505) in Dot Blot.
Dot blot analysis of V3S-0522-YC7505 was performed by coating with recombinant human Met.
The secondary antibody: HRP-anti-human IgG
Figure 3 Human Anti-MET Recombinant Antibody (V3S-0522-YC7505) in Dot Blot.
