Anti-MET Neutralizing Antibody (V3S-0622-YC4914)

Figure 1 Anti-MET Monoclonal Antibody (V3S-0622-YC4914) in SDS-PAGE
Figure 2 Anti-MET Monoclonal Antibody (V3S-0622-YC4914) in SEC-HPLC
Figure 3 Anti-MET Monoclonal Antibody (V3S-0622-YC4914) in WB
Figure 4 Anti-MET Monoclonal Antibody (V3S-0622-YC4914) in ELISA

Datasheet

MSDS

COA

Summary

Description	This product is a human monoclonal antibody that reacts with MET. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality	Monoclonal
Host Species	Human
Target Species	Human
Isotype	IgG

Property

Expression Species	HEK293F or CHO
Conjugation	None
Purity	>95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin	<1 EU/mg, determined by LAL method
Purification	Protein A affinity purified
Sterility	0.2 μM filtered
Formulation	PBS, pH 7.4
Preservation	No preservatives
Stabilizer	No stabilizers
Storage	Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Tested Data

SDS-PAGE

Figure 1 Anti-MET Monoclonal Antibody (V3S-0622-YC4914) in SDS-PAGE

SDS-PAGE analysis of V3S-0622-YC4914 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 1 Anti-MET Monoclonal Antibody (V3S-0622-YC4914) in SDS-PAGE

SEC-HPLC

Figure 2 Anti-MET Monoclonal Antibody (V3S-0622-YC4914) in SEC-HPLC

The purity of V3S-0622-YC4914 was greater than 98% as determined by SEC-HPLC.

Figure 2 Anti-MET Monoclonal Antibody (V3S-0622-YC4914) in SEC-HPLC

Figure 3 Anti-MET Monoclonal Antibody (V3S-0622-YC4914) in WB

Western blot analysis of V3S-0622-YC4914 was performed by loading Recombinant Human MET Protein

Lane 1: Non-Reducing Antigen (1 μg)
Lane 2: Reducing Antigen (1 μg)
V3S-0622-YC4914 antibody incubation concentration: 1 μg/mL.

Figure 3 Anti-MET Monoclonal Antibody (V3S-0622-YC4914) in WB

ELISA

Figure 4 Anti-MET Monoclonal Antibody (V3S-0622-YC4914) in ELISA

ELISA analysis of V3S-0622-YC4914 was performed by coating with Recombinant Human MET Protein.

Figure 4 Anti-MET Monoclonal Antibody (V3S-0622-YC4914) in ELISA

Application	ELISA; WB; FuncS
Application Notes	The antibody is recommended for detection of MET by FuncS assay.

ELISA	Enzyme-Linked Immunosorbent Assay Protocol
WB	Western Blot Protocol
FC	Flow Cytometry Protocol

Target	MET
Alternative Name	HGFR; AUTS9; RCCP2; c-Met; DFNB97
Gene ID	4233
UniProt	P08581
Research Area	Signal Pathway; Epigenetics

Anti-MET Neutralizing Antibody (V3S-0622-YC4914) (CAT#: V3S-0622-YC4914)

Summary

Property

Applications

Protocols

Target

Tested Data

Anti-MET Neutralizing Antibody (V3S-0622-YC4914) (CAT#: V3S-0622-YC4914)

Summary

Property

Applications

Protocols

Target

Tested Data

Isotype Control

Secondary Antibody

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