| Sub Cat | Immunogen | Isotype | Application | |
|---|---|---|---|---|
| V3S-1124-YC1116 | Activated C57BL/6 mouse spleen cells | IgG2b | Block; WB; IP; FuncS; FC | |
| V3S-0822-YC2095 | Activated C57BL/6 mouse spleen cells | IgG2 | Block; FC; IHC-Fr; IP | |
| V3S-0822-YC2098 | IgG2b kappa | FC; FuncS; Neut; IP; IHC-P; IF; IHC-Fr; WB; IHC |
| Description | This product is a rat monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing MHC class II. It can be used for MHC class II detection in Blocking (Block), Western Blot (WB), Immunoprecipitation (IP), Functional Assay (FuncS), Flow Cytometry (FC). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Rat |
| Target Species | Mouse |
| Immunogen | Activated C57BL/6 mouse spleen cells |
| Isotype | IgG2b |
| Conjugation | Unconjugated |
| Purity | >95%, determined by SDS-PAGE |
| Purification | Protein G purified |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | Block; WB; IP; FuncS; FC |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | MHC class II |
| Alternative Name | MHC class II; MHC class II; MHC class II; I-Ab; I-Ad; I-Aq; I-Ed; I-Ek |
| Research Area | Immunology |
