| Description | This product is a mouse monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing macrophage migration inhibitory factor (glycosylation-inhibiting factor). It can be used for MIF detection in Enzyme-Linked Immunosorbent Assay (ELISA), Neutralization Assay (Neut), Western Blot (WB). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Recombinant human MIF with a His tag |
| Isotype | IgG2b kappa |
| Conjugation | Unconjugated |
| Purity | >95%, determined by SDS-PAGE |
| Purification | Affinity purified |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Neut; WB |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | MIF |
| Alternative Name | MIF; macrophage migration inhibitory factor (glycosylation-inhibiting factor); GIF; GLIF; MMIF; macrophage migration inhibitory factor; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase |
| Gene ID | 4282 |
| UniProt | I4AY87 |
| Research Area | Cardiovascular; Immunology |
