| Description | This product is a human monoclonal antibody that reacts with MS4A1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Human lymphoblastoid cell line SB |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | IF; IP; Neut; FuncS; ELISA; FC; WB |
| Application Notes | The antibody is recommended for detection of MS4A1 by IF, IP, Neut, FuncS, ELISA, FC, WB assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC2216) in WB
Western blot analysis of V3S-0622-YC2216 was performed by loading Human CD20 Protein in reduced condition Lane 1, Lane 2 and Lane 3 (0.2 μg, 0.4 μg and 0.8 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC2216 (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
Figure 1 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC2216) in WB
Figure 2 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC2216) in FACS
Flow cytometry analysis of Daudi cells (0.5x106) with purified antibody V3S-0622-YC2216 (5 μl). Cells were blocked with Human TruStain FcXTM and washed with 0.5% BSA-PBS. A FITC Goat anti-human IgG (5 μl) as a secondary antibody.
Figure 2 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC2216) in FACS
