Anti-MS4A1 Neutralizing Antibody (V3S-0622-YC4990) (CAT#: V3S-0622-YC4990)

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  • fig1
    Figure 1 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4990) in ELISA
  • fig1
    Figure 2 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4990) in WB
  • fig1
    Figure 3 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4990) in DB
  • fig1
    Figure 4 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4990) in SDS-PAGE
  • fig1
    Figure 5 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4990) in FACS

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a human monoclonal antibody that reacts with MS4A1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application WB; DB; FC; IF; IP; Neut; FuncS; ELISA; FC; ICC
Application Notes The antibody is recommended for detection of MS4A1 by IF, IP, Neut, FuncS, ELISA, FC, ICC assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target MS4A1
Alternative Name B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
Gene ID 931
UniProt P11836
Research Area Immunology; Stem Cell

Tested Data

ELISA

Figure 1 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4990) in ELISA

ELISA analysis of V3S-0622-YC4990 was performed by coating with Human MS4A1 Protein (His Tag). Then blocked with BSA and incubated with V3S-0622-YC4990. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 1 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4990) in ELISA

WB

Figure 2 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4990) in WB

Western blot analysis of V3S-0622-YC4990 was performed by loading Human MS4A1 Protein (His Tag) in reduced condition Lane 1 (0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC4990 (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.

Figure 2 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4990) in WB

DB

Figure 3 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4990) in DB

Dot Blot analysis of V3S-0622-YC4990 was performed by coating with Human MS4A1 Protein (His Tag).

V3S-0622-YC4990 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)

Figure 3 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4990) in DB

SDS-PAGE

Figure 4 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4990) in SDS-PAGE

SDS-PAGE analysis of V3S-0622-YC4990 in β-mercaptoethanol-reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 4 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4990) in SDS-PAGE

FACS

Figure 5 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4990) in FACS

Flow cytometry analysis of Daudi cells (0.5x106) with purified antibody V3S-0622-YC4990 (5 μl). Cells were blocked with Human TruStain FcXTM and washed with 0.5% BSA-PBS. A FITC Goat anti-human IgG (5 μl) as a secondary antibody.

Figure 5 Anti-MS4A1 Monoclonal Antibody (V3S-0622-YC4990) in FACS

For research use only, not directly for clinical use.
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