Recombinant Anti-NOTCH2 Antibody (V3S-1022-YC2312) (CAT#: V3S-1022-YC2312)

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Datasheet

MSDS

COA

Sub Cat Epitope Affinity  
V3S-1124-YC1594 In the aa 385-389 of human Notch 2. 0.05 nM (Notch2), 0.19 nM (Notch3) Inquiry
V3S-1022-YC2311 In the ECD domain. Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for notch 2. It can be used for notch 2 detection in Flow Cytometry and Enzyme-Linked Immunosorbent Assay. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Human
Target Species Human, Mouse
Immunogen Recombinant Notch2 Fc fusion protein comprising the extracellular ligand binding site of Notch2
Epitope The epitope is in the aa 385-389 of human Notch 2.
Affinity The affinity is in nanomolar range, about 0.05 nM (Notch2), 0.19 nM (Notch3), determined by surface plasmon resonance.
Isotype IgG2 kappa

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application FC; ELISA

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target NOTCH2
Alternative Name NOTCH2; notch 2; hN2; AGS2; HJCYS; neurogenic locus notch homolog protein 2; Notch homolog 2
Gene ID 4853
UniProt Q04721
Research Area Neuroscience; Epigenetics
Related Disease Tumor
For research use only, not directly for clinical use.
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