Recombinant Anti-NOTCH3 Antibody (V3S-0622-YC5137) (CAT#: V3S-0622-YC5137)

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  • fig1
    Figure 1 Anti-Human NOTCH3 Monoclonal Antibody (V3S-0622-YC5137) in FC

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from mouse, which can specifically recognize NOTCH3. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Isotype IgG1 kappa

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application FC; ELISA
Application Notes The antibody is recommended for detection of NOTCH3 by ELISA assay.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target NOTCH3
Alternative Name IMF2; LMNS; CASIL; CADASIL; CADASIL1
Gene ID 4854
UniProt Q9UM47
Related pathway Notch signaling pathway
Research Area Neuroscience; Epigenetics

Tested Data

FC

Figure 1 Anti-Human NOTCH3 Monoclonal Antibody (V3S-0622-YC5137) in FC

Flow cytometry analysis of U87 MG cells with purified antibody V3S-0622-YC5137 (QCAb254, Blue). Cells were blocked with Human TruStain FcXTM and washed with 0.5% BSA-PBS. Cells were then incubated with V3S-0622-YC5137 (5 μl/5x10^5 cells) for 60 min at 4°C. The secondary antibody used was FITC anti-human IgG antibody (3 μl) for 60 min at 4°C.

Figure 1 Anti-Human NOTCH3 Monoclonal Antibody (V3S-0622-YC5137) in FC

For research use only, not directly for clinical use.
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