| Description | This product is a monoclonal antibody derived from mouse, which can specifically recognize NOTCH3. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | FC; ELISA |
| Application Notes | The antibody is recommended for detection of NOTCH3 by ELISA assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-Human NOTCH3 Monoclonal Antibody (V3S-0622-YC5137) in FC
Flow cytometry analysis of U87 MG cells with purified antibody V3S-0622-YC5137 (QCAb254, Blue). Cells were blocked with Human TruStain FcXTM and washed with 0.5% BSA-PBS. Cells were then incubated with V3S-0622-YC5137 (5 μl/5x10^5 cells) for 60 min at 4°C. The secondary antibody used was FITC anti-human IgG antibody (3 μl) for 60 min at 4°C.
Figure 1 Anti-Human NOTCH3 Monoclonal Antibody (V3S-0622-YC5137) in FC
