| Sub Cat | Target Species | Immunogen | Application | Affinity | |
|---|---|---|---|---|---|
| V3S-1124-YC612 | Human, Mouse, Rat | Recombinant human TrkB extracellular domain generated using HEK293-6E cell line expression | ELISA | 3.3 nM | |
| V3S-0522-YC1886 | Human | Recombinant human TrkB extracellular domain generated using HEK293-6E cell line expression | ELISA | 7.5 nM | |
| V3S-0522-YC1887 | Human, Mouse, Rat | Recombinant human TrkB extracellular domain generated using HEK293-6E cell line expression | ELISA | 3.3 nM |
| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Neurotrophic Receptor Tyrosine Kinase 2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human, Mouse, Rat |
| Immunogen | Recombinant human TrkB extracellular domain generated using HEK293-6E cell line expression |
| Epitope | Surface plasmon resonance binding analysis of this antibody with ~80 single point alanine mutants in TrkB-ECD, mostly in the D4-D5-JM domain, identified 6 amino acids as critical residues for binding, although to varying extents (N350>H299, D349>H300, K328, K333). |
| Affinity | KD = 3.3 nM, determined by surface plasmon resonance. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA |
| Application Notes | The antibody is recommended for detection of NTRK2 by ELISA assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | NTRK2 |
| Alternative Name | Neurotrophic Receptor Tyrosine Kinase 2; Neurotrophic Tyrosine Kinase Receptor Type 2; Tropomyosin-Related Kinase B; EC 2.7.10.1; GP145-TrkB; Trk-B; TRKB; Neurotrophic Tyrosine Kinase, Receptor, Type 2 |
| Gene ID | 4915 |
| UniProt | Q16620 |
| Research Area | Neuroscience; Cancer Research |
| Related Disease | Neurological Disorder |
