| Sub Cat | Epitope | |
|---|---|---|
| V3S-1124-YC2212 | Located in the sequence: LKTAAESA. | |
| V3S-0522-YC4847 | Located in the sequence: TPTAWKPN. | |
| V3S-0522-YC4848 | Located in the sequence: KPNYAPANCPKS. |
| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Pseudomonas aeruginosa Fimbrial protein precursor (Pilin). The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Pseudomonas aeruginosa |
| Epitope | Located in the sequence: LKTAAESA. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA |
| Application Notes | The antibody is recommended for detection of P. aeruginosa pilA by ELISA assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | P. aeruginosa pilA |
| Alternative Name | P. aeruginosa pilA; Pseudomonas aeruginosa Fimbrial protein precursor (Pilin); P. aeruginosa; Pseudomonas aeruginosa; Fimbrial protein precursor (Pilin); pilA |
| UniProt | P18774 |
| Research Area | Microbiology |
