Recombinant Anti-PDCD1 Antibody (V3S-0622-YC2362) (CAT#: V3S-0622-YC2362)

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  • fig1
    Figure 1 Anti-PDCD1 Monoclonal Antibody (V3S-0622-YC2362) in WB
  • fig1
    Figure 2 Anti-PDCD1 Monoclonal Antibody (V3S-0622-YC2362) in DB

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a human monoclonal antibody that reacts with PDCD1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application WB; DB; ELISA
Application Notes The antibody is recommended for detection of PDCD1 by ELISA assay.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target PDCD1
Alternative Name PDCD1; programmed cell death 1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; programmed cell death protein 1; protein PD-1; systemic lupus erythematosus susceptibility 2
Gene ID 5133
UniProt Q15116
Research Area Cell Biology

Tested Data

WB

Figure 1 Anti-PDCD1 Monoclonal Antibody (V3S-0622-YC2362) in WB

Western blot analysis of V3S-0622-YC2362 was performed by loading Human PDCD1 Protein (His Tag) in reduced condition (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC2362 (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.

Figure 1 Anti-PDCD1 Monoclonal Antibody (V3S-0622-YC2362) in WB

DB

Figure 2 Anti-PDCD1 Monoclonal Antibody (V3S-0622-YC2362) in DB

Dot Blot analysis of V3S-0622-YC2362 was performed by coating with Human PDCD1 Protein (His Tag).

V3S-0622-YC2362 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)

Figure 2 Anti-PDCD1 Monoclonal Antibody (V3S-0622-YC2362) in DB

For research use only, not directly for clinical use.
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