| Description | It is an antibody clone R5.015 specific for Reticulocyte-binding protein homolog 5 (RH5), derived from human. It binds close to the C-terminus of RH5, through interactions with the C-termini of helices 5 and 7 of RH5. This antibody is not growth inhibitory and also prevents the binding of CyRPA to RH5. This product has been determined by Enzyme-linked immunosorbent assay and Surface plasmon resonance. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Plasmodium falciparum |
| Epitope | R5.015 binds close to the C-terminus of RH5, through interactions with the C-termini of helices 5 and 7 of RH5. |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; SPR |
| Application Notes | ELISA: 50 μL of mAb at 10 μg/mL, diluted in PBS-casein. mAb solution was incubated for 60 min at room temperature. Surface plasmon resonance (SPR): Purified mAb was immobilized on a protein G chip through a 30 s injection of 20 nM mAb. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | PfRH5 |
| Alternative Name | Reticulocyte-binding protein homolog 5 |
| Research Area | Infectious Disease |
| Related Disease | Malaria |
