Description | This product is a mouse monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing Phosphatidylinositol 4,5-biphosphate. It can be used for PIP2 detection in Immunocytochemistry (ICC), Immunofluorescence (IF), Enzyme-Linked Immunosorbent Assay (ELISA), Neutralization Assay (Neut), Immunoprecipitation (IP). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
Clonality | Monoclonal |
Host Species | Mouse |
Target Species | Mouse, Rat, Human |
Immunogen | Liposomes (prepared in PBS with lipid A, phosphatidylcholine (PC) and cholesterol) containing synthetic di-palmitoyl PtdIns(4,5)P2. |
Isotype | IgM |
Conjugation | Unconjugated |
Purity | >95%, determined by SDS-PAGE |
Purification | Immunogen affinity purified |
Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
Application | ICC; IF; ELISA; Neut; IP |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | PIP2 |
Alternative Name | PIP2; Phosphatidylinositol 4;5-biphosphate |
Research Area | Signal Pathway |