| Description | This product is a human monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing human prostate-specific membrane antigen (PSMA). It can be used for PSMA detection in Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry (FC) and Antibody-dependent Cellular Cytotoxicity Assay (ADCC). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A or G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | WB; DB; ELISA; FC; ADCC |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-PSMA Monoclonal Antibody (V3S-0822-YC3122) in WB
Western blot analysis of V3S-0822-YC3122 (2 μg/mL) was performed by loading Human PSMA Protein (His Tag). The HRP-Anti-Human IgG (H+L) (1: 6000) as a secondary antibody.
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.2 μg)
Lane 3: Reducing Antigen (0.4 μg)
Figure 1 Anti-PSMA Monoclonal Antibody (V3S-0822-YC3122) in WB
Figure 2 Anti-PSMA Monoclonal Antibody (V3S-0822-YC3122) in DB
Dot Blot analysis of V3S-0822-YC3122 (2 μg/mL) was performed by coating with Human PSMA Protein (His Tag). The HRP-Anti-Human IgG (H+L) (1: 6000) as a secondary antibody.
Figure 2 Anti-PSMA Monoclonal Antibody (V3S-0822-YC3122) in DB
Figure 3 Anti-PSMA Monoclonal Antibody (V3S-0822-YC3122) in ELISA
ELISA analysis of V3S-0822-YC3122 was performed by coating with Recombinant Human PSMA Protein (His Tag). Then blocked with BSA, and incubated with V3S-0822-YC3122. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1:5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-PSMA Monoclonal Antibody (V3S-0822-YC3122) in ELISA
