| Description | This product is a monoclonal antibody derived from mouse, which can specifically recognize RSPO1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Recombinant human RSPO1 protein amino acids 31-263 |
| Epitope | within the residues 31 to 263 |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; Block; IP |
| Application Notes | The antibody is recommended for detection of RSPO1 by WB, ELISA, Block, IP assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Recombinant Mouse Anti-RSPO1 Antibody (V3S-0622-YC5257) (V3S-0622-YC5257) in WB
Western blot analysis of V3S-0622-YC5257 was performed by loading recombinant human RSPO1 .
V3S-0622-YC5257 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat anti-Mouse IgG
Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)
Figure 1 Recombinant Mouse Anti-RSPO1 Antibody (V3S-0622-YC5257) (V3S-0622-YC5257) in WB
Figure 2 Recombinant Mouse Anti-RSPO1 Antibody (V3S-0622-YC5257) (V3S-0622-YC5257) in ELISA
ELISA analysis of V3S-0622-YC5257 was performed by coating with recombinant human RSPO1 protein. Then blocked with BSA and incubated with Mouse Anti-RSPO1 Recombinant Antibody (V3S-0622-YC5257). The Goat Anti-Mouse IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Recombinant Mouse Anti-RSPO1 Antibody (V3S-0622-YC5257) (V3S-0622-YC5257) in ELISA
