Anti-RSPO1 (aa 31-263) Neutralizing Antibody (V3S-0622-YC5257) (CAT#: V3S-0622-YC5257)

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  • fig1
    Figure 1 Recombinant Mouse Anti-RSPO1 Antibody (V3S-0622-YC5257) (V3S-0622-YC5257) in WB
  • fig1
    Figure 2 Recombinant Mouse Anti-RSPO1 Antibody (V3S-0622-YC5257) (V3S-0622-YC5257) in ELISA






Description This product is a monoclonal antibody derived from mouse, which can specifically recognize RSPO1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Immunogen Recombinant human RSPO1 protein amino acids 31-263
Epitope within the residues 31 to 263
Isotype IgG


Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.


Application WB; ELISA; Block; IP
Application Notes The antibody is recommended for detection of RSPO1 by WB, ELISA, Block, IP assays.


ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol


Target RSPO1
Alternative Name RSPO1; R-spondin 1; RSPO; CRISTIN3; R-spondin-1; R-spondin homolog; roof plate-specific spondin-1; anti-RSPO1
Gene ID 284654
UniProt Q2MKA7
Related pathway Wnt signaling pathway
Research Area Stem Cell; Developmental Biology

Tested Data


Figure 1 Recombinant Mouse Anti-RSPO1 Antibody (V3S-0622-YC5257) (V3S-0622-YC5257) in WB

Western blot analysis of V3S-0622-YC5257 was performed by loading recombinant human RSPO1 .

V3S-0622-YC5257 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat anti-Mouse IgG

Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)

Figure 1 Recombinant Mouse Anti-RSPO1 Antibody (V3S-0622-YC5257) (V3S-0622-YC5257) in WB


Figure 2 Recombinant Mouse Anti-RSPO1 Antibody (V3S-0622-YC5257) (V3S-0622-YC5257) in ELISA

ELISA analysis of V3S-0622-YC5257 was performed by coating with recombinant human RSPO1 protein. Then blocked with BSA and incubated with Mouse Anti-RSPO1 Recombinant Antibody (V3S-0622-YC5257). The Goat Anti-Mouse IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Recombinant Mouse Anti-RSPO1 Antibody (V3S-0622-YC5257) (V3S-0622-YC5257) in ELISA

For research use only, not directly for clinical use.
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