| Description | This product is a human monoclonal antibody that reacts with RSPO3. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Mouse, Cynomolgus |
| Immunogen | Recombinant human RSP03 |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; Neut; FC; IHC; IP |
| Application Notes | The antibody is recommended for detection of RSPO3 by WB, ELISA, Neut, FC, IHC, IP assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | RSPO3 |
| Alternative Name | RSPO3; R-spondin 3; PWTSR; THSD2; CRISTIN1; R-spondin-3; R-spondin 3 homolog; roof plate-specific spondin-3; protein with TSP type-1 repeat; thrombospondin; type I; domain containing 2; thrombospondin type-1 domain-containing protein 2; anti-RSPO3 |
| Gene ID | 84870 |
| UniProt | Q9BXY4 |
| Related pathway | Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathway |
| Research Area | Stem Cell; Developmental Biology |
