| Description | This product is a mouse antibody to RSV F. The antibody recognizes both pre-fusion and post-fusion RSV F and binds strongly to a previously unidentified neutralizing epitope positioned between antigenic sites II and IV in the F1 fragment. The antibody has a high affinity for RSV F NP, with a KD value of 0.07 nM. Additionally, it binds to the site II peptide (residues 254-278) with a KD value of 12.5 nM. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Respiratory Syncytial Virus (RSV) |
| Immunogen | RSV F NP protein |
| Epitope | NSELLSLINDMPITNDQKKLMSNNV (AA 254-278) |
| IC50 | 1078 ng/mL |
| Affinity | KD = 0.07 nM (RSV F NP vaccine) KD = 0.34 nM (post-fusion RSV F) KD = 7.09 nM (pre-fusion RSV F) |
| Isotype | Mouse IgG2a, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Neut |
| Application Notes | Characterization of RSV F-specific monoclonal antibody binding to RSV with an IC₅₀ of 1.078 μg/mL. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | RSV F |
| Alternative Name | Human respiratory syncytial virus fusion protein; Human respiratory syncytial virus; fusion protein; F protein; RSV fusion (F) glycoprotein; fusion glycoprotein |
| Research Area | Microbiology |
