| Description | The antibody S3H3 is a unique human neutralizing antibody that binds to the SD1 region of the SARS-CoV-2 spike protein. It is an antibody that is able to cross-neutralize major variants of concern including Omicron and it remains highly effective against Omicron. This product has been determined using Enzyme-linked immunosorbent assay, Bio-layer interferometry and Neutralization assay. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Cross Reactivity | Wuhan-Hu-1 strain, Delta, Omicron |
| Epitope | It binds to the SD1 region of the WT S protein. |
| IC50 | 82.2 ng/mL (Wuhan-Hu-1 strain) 39.3 ng/mL (Delta) 53.3 ng/mL (Omicron) 0.548 nM (Wuhan-Hu-1 strain) 0.262 nM (Delta) 0.355 nM (Omicron) |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; BLI; Neut |
| Application Notes | ELISA: After washing with PBST, the plates were incubated with 50 ng per well of each of the anti-SARS-CoV-2 mAbs at 37°C for 2 h. Neutralization assay (Neut): All mAbs were fourfold serially diluted and tested by a pseudovirus Neutralization assayralization assay with human ACE2-overexpressing HEK 293T cells. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SARS-CoV-2 S |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 Spike S1 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
