| Description | The antibody Fab14 binds to SARS-CoV-2 spike protein. It can only bind RBD in the up confirmation as, in the down conformation, it would clash with an adjacent RBD domain. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | Spike protein |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | The RBD proteins were coated on Corning high binding assay plates with a concentration of 2 μg/ml at 4°C overnight and blocked with 5% skim milk at 37°C for 2 h. Serially diluted antibodies were added at a volume of 100 μl per well for incubation at 37°C for 2 h. The anti_x0002_human IgG Fab2 HRP-conjugated antibody was diluted 1:5000 and added at a volume of 100 μl per well for incubation at 37°C for 1 h. The plates were washed 5 times with PBST between incubation steps. TMB substrate was added 100 μl per well for color development for 3 min and 2 M H2SO4 was added 50 μl per well to stop the reaction. The OD450 was read by a SpectraMax microplate reader with data collected by SoftMax Pro version 6.5.1. The data points were plotted using GraphPad Prism8, and the EC50 values were calculated using a three-parameter nonlinear model. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
