| Description | Cv2.1169, expressed as a human neutralizing IgG1 antibody, is one of the strongest ADCP-inducer among all the SARS-CoV-2 Spike mAbs. Cv2.1169 has high affinity for SARS-CoV-2 S1 and potent neutralizing ability against Wuhan, D614G, Alpha, Beta, Gamma, and Delta variants. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 (Wuhan, D614G, Alpha, Beta, Gamma and Delta) |
| Epitope | The epitope comprises the RBD segments 417-421, 455-458, 473-478, and 484-493. |
| Affinity | 1.44 nM |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; FC; Neut; SPR; ADCP |
| Application Notes | This antibody was evaluated by ELISA, Flow cytometry (FC), Neutralization assay and Surface plasmon resonance (SPR). The ELISA was done by using 70 nM Cv2.1169 IgG1 antibody. For Flow cytometry, the transfected cells were incubated with 1 µg/mL Cv2.1169 IgG at 4°C for 30 min. The Neutralization ability of Cv2.1169 to S-Fuse was measured by Neutralization assay with IC50 value of 0.95 ng/mL. The affinity of antibody was determined by SPR with 10 μg/mL IgG injected over sensor surfaces. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SARS-CoV-2 S1 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 Spike S1 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
