| Description | This human antibody is specific for Spike S1 protein of SARS-CoV-2, termed as Ab326. Ab326 belongs to the Class II type antibody that binds from the upper side of RBD. It shows an neutralizing ability against SARS-CoV-2 Wuhan. But Ab326 could not neutralize the Beta and Gamma variants due to E484K mutation. This product has been determined by Enzyme-linked immunosorbent assay, Bio-layer interferometry, Surface plasmon resonance and Neutralization assay. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 (Wuhan, Alpha, Delta) |
| Epitope | Ab326 binds from the upper side of RBD. |
| Affinity | 0.346 pM |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; SRP; BLI; Neut |
| Application Notes | Bio-layer interferometry (BLI): The sensors were saturated with the first antibody (60 μg/mL) for 600 s. Surface plasmon resonance (SPR): Ab326 was performed at a different concentrations (0, 62.5, 125, 250, 500, 1000 ng/mL). Neutralization assay: Ab326 showed an IC50 against the SARS-CoV-2 variant Wuhan, Alpha and Delta variants of 59.5, 3.0 and 58.7 ng/mL, respectively. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SARS-CoV-2 S1 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 Spike S1 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
