| Description | This is a anti-SARS-CoV-2 Spike S1 mouse antibody 17B10 recoginzing receptor-binding domain ridge. 17B10 broadly neutralizes infectious SARS-CoV-2 from ancestral strains to Delta variants. It is highly potent in microneutralization assay against infectious SARS-CoV-2 variants. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | SARS-CoV-2 |
| Cross Reactivity | Alpha, Lambda, Delta |
| Immunogen | The RBD (residues 319-533) of Wuhan-Hu-1 strain (GenBank: NC_045512.2) with a 6xHis-tag. |
| Epitope | The binding surface of 17B10 is formed primarily by residues 455−458 and residues 473−493, particularly with residues 458, 477, 478, 486, 487, and 489 as the key contacting sites |
| Neutralization Mechanism | The antibody targets the tip of the receptor binding motif in the RBD-up conformation. |
| IC50 | < 0.01 µg/mL (Lambda (C.37), Beta (B.1.351), Gamma (P.1)) 0.01-0.1 µg/mL (B.1.5, Zeta (P.2), R.1, Kappa (B.1.617.1)) 0.1-1 µg/mL (Delta (B.1.617.2)) >20 µg/mL (Omicron (B.1.1.529)) |
| Affinity | < 1 pM |
| Isotype | Mouse IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; BLI; Neut |
| Application Notes | ELISA: Serially diluted mouse mAbs were then added and were incubated at room temperature for 1 h. Bio-layer interferometry (BLI): The RBD loaded SA biosensors were dipped in 17B10 mAb (50 µg/mL in 1X Kinetics Buffer) for 600 s. Neutralization assay (Neut): Serially diluted mAbs were incubated with SARS-CoV-2 pseudovirus particles bearing the original D614 spike at 37°C for 1 h and were then incubated with HEK293-ACE2 target cells in Poly-D-lysine coated 384-well plates for 2 days. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SARS-CoV-2 S1 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 Spike S1 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
