| Description | The antibody ZCB11 is a human mAb specific for SARS-CoV-2 Spike S1 protein. It targets viral receptor-binding domain specifically and neutralizes all SARS-CoV-2 variants of concern, especially with great potency against authentic Omicron and Delta variants. It is a promising broadly neutralizing antibody for biomedical interventions against pandemic SARS-CoV-2. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Cross Reactivity | Omicron and Delta variants of SARS-CoV-2 |
| Epitope | Receptor-binding domain (RBD) |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | The recombinant RBD and trimeric spike proteins derived from SARS-CoV-2 were diluted to final concentrations of 1 μg/mL, then coated onto 96-well plates and incubated at 4°C overnight. Plates were washed with PBST and blocked with blocking buffer at 37°C for 1 h. Serially diluted plasma samples or isolated monoclonal antibodies were added to the plates and incubated at 37°C for 1 h. Wells were then incubated with a secondary goat anti-human IgG labeled with HRP TMB substrate. OD at 450 nm was measured by SkanIt RE6.1 with VARIOSKAN Lux. Serially diluted plasma from healthy individuals or previously published monoclonal antibodies against SARS-CoV-2 were used as negative controls. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
