| Description | The antibody MW06 is a human mAb specific for SARS-CoV-2 Spike S1 protein. It is a fully human cross-reactive mAb, which binds to both SARS-CoV-2 and SARS-CoV spike receptor-binding domain and disrupts their interaction with angiotensin-converting enzyme 2 (ACE2) receptors. It recognizes a conserved epitope on SARS-CoV-2, which provides insights for the development of a universal antibody-based therapy against SARS-related coronavirus and emerging variant strains, and may be an effective anti-SARS-CoV-2 agent. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Cross Reactivity | SARS-related coronavirus |
| Epitope | Receptor-binding domain (RBD) |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | To access the binding of mAbs to recombinant proteins (SARS-CoV-2 RBD, SARS-CoV-2 RBD mutants, SARS-CoV RBD), recombinant proteins were coated on 96-well ELISA plates at 10 µg/ml in 100 µL at 4°C for overnight. After blocking with 5% bovine serum albumin in PBS, serially diluted mAbs were added to the plates and incubated at 37°C for 1 hour. Plates were washed and second_x0002_ary Ab Goat Anti-Human IgG Fc-HRP was added. TMB was used for color development and absorbance at 450 nm was measured using a microplate reader. For the RBD/ACE2-hFc blocking assay, ACE2-hFc recombinant protein was coated on a 96-well ELISA plate at 0.75 µg/ml in 100 µL at 4°C for overnight. The equivalent volumes (100 µL + 100 µL) of pre-incubated RBD-mFc/mAb complex were added to the plates and incubated for 1 hour at 37°C. Plates were washed and secondary Ab Goat Anti-Mouse IgG Fc-HRP was added. TMB was used for color development and absorbance at 450 nm was measured using a microplate reader. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
