| Description | The anti-SARS-CoV-2 antibody is a rabbit IgG, clone 9H1X. This product is a recombinant antibody provided by Creative Biolabs. It interacts with the receptor-binding motif (RBM) region of the receptor-binding domain on the spike protein and by directly competing with ACE2, it blocks the binding of the virus to the receptor and achieves neutralization. |
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Target Species | SARS-CoV-2 |
| Epitope | Receptor-binding domain (RBD) |
| Isotype | IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | ELISA plates were coated with 25 μL of 1 μg/mL WT, Delta and Omicron spike ECD or RBD proteins and incubated overnight at 4°C. After washing and blocking, 25 μL 9H1 RmAbs of gradient concentrations were added to each well and incubated for 1 h. Then, goat anti-rabbit IgG-HRP was added and incubated for 1 h at room temperature. Thermo TMB substrate was then added and plates were kept in the dark at room temperature. Finally, the OD of 450 nm was measured using the Epoch micro_x0002_plate spectrophotometer. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
