Description | The antibody GAR15 is a human mAb specific for SARS-CoV-2 Spike S1 protein. The IC50 value of antibody against SARS-CoV-2 is 157 ng/mL. The KD of antibody for RBD is 1.30 nM. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | SARS-CoV-2 |
Epitope | Receptor-binding domain (RBD) |
Affinity | 1.30 nM |
Isotype | IgG1 |
Expression Species | HEK293F or CHO cell line |
Conjugation | Unconjugated |
Purity | >95% |
Endotoxin | <1 EU/mg |
Form | Liquid |
Purification | Protein A purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
Application | ELISA, Neut |
Application Notes | The IC50 value of GAR15 is 157 ng/ml. Maxisorp plates were coated with SARS-CoV-2 RBD at 2 µg/ml in carbonate coating buffer overnight at 4°C. The following day, plates were washed twice with PBS and blocked in 4% milk in PBS-T for 2 h. Plates were washed 3x with PBS-T and incubated with the antibody GAR15 diluted to 100 µg/ml in PBS for 1 h. Plates were then washed 3x with PBS-T and incubated with HRP-conjugated goat Anti-human IgG antibody for 1 h. Plates were finally washed 2x with PBS-T and 1x with PBS and incubated with TMB substrate until the reaction was quenched with HCl. Plates were read at Abs 450 nm. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Western Blot Protocol |
Target | SARS-CoV-2 |
Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
Research Area | Coronavirus Disease 2019 |
Related Disease | Coronavirus Disease 2019 |