| Description | The anti-SARS-CoV-2 antibody is a mouse IgG, clone SW186. This product is a recombinant antibody provided by Creative Biolabs. SW186 can neutralize major SARS-CoV-2 variants of concern, including Beta, Delta, and Omicron, as well as SARS-CoV-1. It is a broadly neutralizing antibody against SARS coronaviruses and binds to a unique and conserved epitope of the spike protein. It neutralizes diverse SARS coronaviruses by binding to a conserved RBD epitope, which could serve as a target for further antibody development. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | SARS-CoV-2 |
| Cross Reactivity | Beta, Delta, Omicron and SARSCoV-1 |
| Epitope | Receptor-binding domain (RBD) |
| Isotype | IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | To measure the binding of antibodies with target antigens, 50 ng of S-ecto or RBD was coated on each well of the 96-well ELISA plate at 4°C overnight. After washing with PBST, plates were incu_x0002_bated with a blocking buffer at room tem_x0002_perature for 2 hours. Antibodies at different dilutions were added to the plates and incubated at room temperature for 1.5 hours. After washing with PBST, plates were incubated with anti-human-IgG-HRP for 45 min. Plates were washed with PBST 5 times between two steps. Finally, TMB substrate solution was incubated with the plate for 15 min before addition of a stop solution. Absorbance at 450nm was measured using CLARIOstar Plus Microplate Reader. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
