| Description | The antibody 10-28 is a human mAb specific for SARS-CoV-2 Spike S1 protein. mAb exhibits binding to three S proteins derived from Asian bat sarbecoviruses. For the antibody crystal structure, antibody-RBD side-chain interactions were well defined at 3.2 Å resolution. Interactions were mediated by antibody CDR loops H1, H3, L1, and L3, which predominantly contacted α3, but also α2 and the β2-α3, α4-α5 and α5-β4 loops of the RBD. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Cross Reactivity | Asian bat sarbecovirus |
| Epitope | Antibody 10-28 predominantly contacted α3, but also α2 and the β2-α3, α4-α5 and α5-β4 loops of the RBD |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | 50 ng per well of antigen (S trimer or RBD protein) was coated onto ELISA plates at 4°C overnight. The ELISA plates were then blocked with 300 μL blocking buffer consisting of PBS with 1% BSA and 10% bovine calf serum at 37°C for 2 hours. Afterwards, 100 μL of serially diluted antibodies were added and then incubated at 37°C for 1 hour. Next, 100 μL of 10,000-fold diluted Peroxidase AffiniPure goat anti-human IgG Fcγ fragment specific antibody was added into each well and incubated for an other 1 hour at 37°C. The plates were washed between each step with PBS-T. Finally, TMB substrate was added and incubated before the reaction was stopped using 1 M sulfuric acid. Absorbance was measured at 450 nm. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
