| Description | The antibody XG014 is a human mAb specific for SARS-CoV-2 Spike S1 protein. The antibody is a receptorbinding domain (RBD)-binding antibody, which potently neutralizes β-coronavirus lineage B (β-CoV-B), including SARS-CoV-2, its circulating variants, SARS-CoV and bat SARSr-CoV WIV1. It recognizes a conserved epitope outside the ACE2 binding site and completely locks RBD in the non-functional "down" conformation. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Cross Reactivity | Betacoronavirus |
| Epitope | Receptor-binding domain (RBD) |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | ELISA plates were coated with 10 µg/mL of S-ECD or RBD of wild-type SARS-CoV-2, or its variants, including B.1.1.7, B.1.351 and P.1 in PBS overnight at 4°C. Plates were then blocked with 2% BSA in PBS. Antibodies were three-fold serially diluted with a maximum concentration of 10 µg/mL for eight dilutions and incubated for one hour at room temperature. Detection was performed after incubating with HRP-conjugated goat anti-human IgG for one hour at room temperature. The area under the curve (AUC) was calculated by using PRISM software. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
