Anti-SARS-CoV-2 Spike S1 (RBD) Neutralizing Antibody (V3S-0623-XY110) (CAT#: V3S-0623-XY110)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description The antibody XG005 is a human mAb specific for SARS-CoV-2 Spike S1 protein. XG005 blocked the interaction of SARS-CoV-2 RBD with the cellular receptor ACE2. The antibody-RBD interaction focused on two loop regions of RBD, with the latter overlapping with the RBM. And the antibody induces the SARS-CoV-2 S conformational equilibrium toward opened RBDs.
Clonality Monoclonal
Host Species Human
Target Species SARS-CoV-2
Epitope Receptor-binding domain (RBD)
Isotype IgG1

Property

Expression Species HEK293F or CHO cell line
Conjugation Unconjugated
Purity >95%
Endotoxin <1 EU/mg
Form Liquid
Purification Protein A purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application ELISA, Neut
Application Notes ELISA plates were coated with 10 µg/mL of S-ECD or RBD of wild-type SARS-CoV-2, or its variants, including B.1.1.7, B.1.351 and P.1 in PBS overnight at 4°C. Plates were then blocked with 2% BSA in PBS. Antibodies were three-fold serially diluted with a maximum concentration of 10 µg/mL for eight dilutions and incubated for one hour at room temperature. Detection was performed after incubating with HRP-conjugated goat anti-human IgG for one hour at room temperature. The area under the curve (AUC) was calculated by using PRISM software.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Western Blot Protocol

Target

Target SARS-CoV-2
Alternative Name Severe acute respiratory syndrome coronavirus 2
Research Area Coronavirus Disease 2019
Related Disease Coronavirus Disease 2019
For research use only, not directly for clinical use.
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